pWY026 pEF1a_MTS-LplA-HA-GFP

LOCUS       pWY026 pEF1a_MTS-LplA-HA-GFP               9909 bp DNA     circular SYN 13-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     491..510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(729..833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     901..919
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(957..979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     981..1005
                     /label=Seq-primer F
     primer_bind     1056..1078
                     /label=Primer 1
     primer_bind     1097..1136
                     /label=Fragment.FOR
     misc_feature    1117..2580
                     /standard_name="L1: oriT traJ + CEN ARS HIS (concatenated 
                     sequence 2)"
                     /label=L1-pICH47732
                     /note="B/3"
     CDS             complement(1194..1565)
                     /codon_start=1
                     /gene="traJ"
                     /standard_name="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            1598..1707
                     /standard_name="incP origin of transfer oriT"
                     /label=incP origin of transfer oriT
                     /note="incP origin of transfer"
     misc_feature    1843..1846
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    1890..2393
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     regulatory      1890..2393
                     /standard_name="CEN/ARS"
                     /label=CEN/ARS
     primer_bind     complement(1984..2015)
                     /label=Primer 3
     misc_feature    2103
                     /standard_name="A"
                     /label=A
                     /note="Geneious type: Editing History Replacement"
     promoter        2394..2580
                     /gene="S. cerevisiae HIS3"
                     /standard_name="HIS3 promoter"
                     /label=HIS3 promoter
     primer_bind     2474..2500
                     /label=S11F
     primer_bind     2561..2602
                     /label=C18F
     promoter        2581..3180
                     /label=pro-EF1a
     misc_feature    2849
                     /label=T
     primer_bind     3051..3072
                     /label=S13F
     primer_bind     3109..3136
                     /label=W3a
     primer_bind     complement(3140..3189)
                     /label=Fragment 1.REV
     primer_bind     3158..3183
                     /label=GapC3-MTS-F
     primer_bind     3162..3183
                     /label=LplA-F2
     primer_bind     3172..3202
                     /label=C19F
     CDS             3181..3432
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             3739..4632
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     primer_bind     3739..3763
                     /label=W3b
     misc_feature    4043
                     /label=T to G mutation
     primer_bind     4412..4437
                     /label=W3c
     primer_bind     complement(4607..4642)
                     /label=C19R
     primer_bind     4624..4672
                     /label=C20F
     misc_feature    4633..4873
                     /label=fcpA terminator
     primer_bind     complement(4848..4886)
                     /label=C20R
     primer_bind     4866..4891
                     /label=seq-p26-F2
     primer_bind     4869..4900
                     /label=C23F
     misc_feature    4884..5292
                     /label=Feature 25
     primer_bind     4901..4914
                     /label=seq-p26-F1
     misc_feature    4957..5105
                     /label=tetra-LexA operator
     primer_bind     5099..5127
                     /label=S18F
     promoter        5188..5234
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     5247..5266
                     /label=35S-F
     primer_bind     complement(5268..5292)
                     /label=35S-lplA-R
     primer_bind     complement(5271..5292)
                     /label=C23R
     primer_bind     5272..5295
                     /label=LplA-35s-F
     primer_bind     5273..5312
                     /label=Gapc3-MTS-F2
     transit_peptide join(5293..5345,5574..5604)
                     /gene="TPI-GapC3"
                     /note="contains a putative cleavage site"
     CDS             join(5293..5345,5574..5604)
                     /codon_start=1
                     /gene="TPI-GapC3"
                     /product="triosephosphateisomerase/glyceraldehyde-3-phospha
                     te dehydrogenase precursor"
                     /label=TPI-GapC3
                     /protein_id="AAF34330.1"
                     /translation="MLASSRTAAASVQRMSSRAFHASSLTEA"
     mRNA            join(5293..5345,5574..5604)
                     /gene="TPI-GapC3"
                     /product="triosephosphate isomerase 
                     +glyceraldehyde-3-phosphate dehydrogenase precursor"
                     /label=TPI-GapC3 mRNA
     gene            5293..5604
                     /gene="TPI-GapC3"
                     /label=TPI-GapC3
     primer_bind     5293..5312
                     /label=GapC3-MTS-F
     misc_feature    5293..5295
                     /label=HSP70-signal peptide
     intron          5346..5573
     primer_bind     5585..5625
                     /label=LplA-F1
     primer_bind     complement(5585..5604)
                     /label=GapC3-MTS-R
     CDS             5605..6618
                     /codon_start=1
                     /label=LPLA
                     /translation="GSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTVV
                     IGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTISTSI
                     VLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLA
                     NYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEAEIIS
                     PNKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQV
                     FTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR"
     primer_bind     5605..5631
                     /label=NcoI-F
     primer_bind     5605..5622
                     /label=LplA-35s-F
     primer_bind     5605..5622
                     /label=LplA-F2
     primer_bind     complement(6319..6345)
                     /label=LplA-N-R
     misc_feature    6346..6618
                     /label=C domain
     primer_bind     6346..6369
                     /label=lplA-C-F
     primer_bind     complement(6599..6638)
                     /label=LplA-R1
     primer_bind     complement(6600..6618)
                     /label=LplA-his R
     primer_bind     complement(6601..6620)
                     /label=XhoI-R
     primer_bind     complement(6618..6644)
                     /label=Primer 2
     primer_bind     complement(6618..6644)
                     /label=Fragment.REV (2)
     CDS             6619..6645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6619..6638
                     /label=BB
     CDS             6619..6638
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVP"
     primer_bind     6619..6637
                     /label=vector-lpla-r1
     primer_bind     6619..6637
                     /label=Vector-lplA-R
     CDS             6649..6675
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6678..6722)
                     /label=Primer 2
     primer_bind     complement(6678..6705)
                     /label=Fragment.REV (2)
     CDS             6679..6705
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6695..6714)
                     /label=V-PEF1A-R
     misc_feature    6706..7422
                     /label=eGFP
     primer_bind     6706..6723
                     /label=Fragment.FOR
     primer_bind     complement(6743..6762)
                     /label=GEP-IN-R
     misc_feature    7321..7323
                     /label=from K(aag) to A(gcc)
     primer_bind     complement(7399..7437)
                     /label=C24R
     primer_bind     complement(7420..7438)
                     /label=C23R
     primer_bind     complement(7437..7453)
                     /label=Fragment.REV (2)
     primer_bind     complement(7522..7548)
                     /label=seq-p26-R1
     primer_bind     complement(7522..7548)
                     /label=Seq-Primer R
     misc_feature    7557..7797
                     /label=fcpA terminator
     primer_bind     7557..7596
                     /label=C20F
     primer_bind     complement(7772..7798)
                     /label=C20R
     misc_feature    7798..8036
                     /label=pfcpB
     primer_bind     7798..7819
                     /label=Primer 2
     misc_feature    8055..8477
                     /label=bsr
     primer_bind     8491..8531
                     /label=C20F
     misc_feature    8492..8732
                     /label=fcpA terminator
     primer_bind     complement(8707..8732)
                     /label=C20R
     primer_bind     complement(8741..8760)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8742..8760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8847..8864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9018..9606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9098..9117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9777..9909,1..728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
       61 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
      361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
      421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
      481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      841 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      901 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
      961 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     1021 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     1081 gcatcagagc agattgtact gagagtgcac catatggcct cttcttgatg gagcgcatgg
     1141 ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc
     1201 tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg
     1261 atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg
     1321 gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc
     1381 tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc
     1441 aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg
     1501 tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca
     1561 gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga
     1621 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
     1681 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
     1741 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
     1801 atcgctataa tgaccccgaa gcagggttat gcagcggaag atttgtatgt gtccagcaaa
     1861 gtagtagctg ctccagttct cccgcgagca tcacgtgcta taaaaataat tataatttaa
     1921 attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa
     1981 atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct
     2041 tttaccttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta
     2101 gatgaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata
     2161 tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa
     2221 attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat
     2281 ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa
     2341 attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atcctagtac
     2401 actctatatt tttttatgcc tcggtaatga ttttcatttt ttttttccac ctagcggatg
     2461 actctttttt tttcttagcg attggcatta tcacataatg aattatacat tatataaagt
     2521 aatgtgattt cttcgaagaa tatactaaaa aatgagcagg caagataaac gaaggcaaag
     2581 cgaaacgaat agaagctccc cgaggtcggg tgttgtttgg gaggttcatg gtggtttcgg
     2641 tgtcgcttgc tcgctcgctc gttcgcagtg acagacagtt cgtgagacac gagaaccgtt
     2701 ggcgtccgag ttcgggtgcc gcatttcgtc gtctccacga ttcaattctt gcccatcaga
     2761 cgagtcccga attccgtgac tctggatgcg atttactgtc caaacgaaac ccaacgattc
     2821 cgtacgttgt tttctatttt acagtgagac ttcgatacca ccgtacaacc atcgttcgtg
     2881 taccgtctgg tagtcccacg tgtcgacaac gtgtggctct ggaccgatga gttgtttgcc
     2941 gttcggaaac gagcagtacc aaggaattca cagaaacaca gcccatgtaa cacaacgacc
     3001 gcgaatcgtt tcggtgctct cgcttcgcgt acgggcgggc ggtccccccc aagcagcgag
     3061 aggagtccgc agcgtcatag ttgcattccg gggcccactc gcgttgttca ctctctcgtc
     3121 tagtagagaa acttccatcg gatcgtatca taatattgta tcgtataata tcacgtaatc
     3181 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     3241 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     3301 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     3361 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     3421 cgtgtggctg ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag
     3481 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     3541 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     3601 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     3661 ggaattgacg agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg
     3721 ccaagcccgc tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc
     3781 gaccagatgg tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat
     3841 cctaccagac ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg
     3901 gagctggttc acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc
     3961 catgatcagg tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc
     4021 tggcgctcca tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg
     4081 aaccagggaa aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca
     4141 tctcggttcc gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt
     4201 ttgcttaatt ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag
     4261 gaccatatcc accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag
     4321 gcaggcctga ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc
     4381 cacatcaggc acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac
     4441 gtggtgcccc tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc
     4501 actagccgtg gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc
     4561 tctacttcat cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct
     4621 gccacagtct gaccttcctt aaaaatttaa ttttcattag ttgcagtcac tccgctttgg
     4681 tttcacagtc aggaataaca ctagctcgtc ttcaccatgg atgccaatct cgcctattca
     4741 tggtgtataa aagttcaaca tccaaagcta gaacttttgg aaagagaaag aatatccgaa
     4801 tagggcacgg cgtgccgtat tgttggagtg gactagcaga aagtgaggaa ggcacaggat
     4861 gagttttctc gagagctcgg tactcgacag cttgcatgcc gcttgggctg caggtcgagg
     4921 ctaaaaaact aatcgcatta tcatcccctc gacgtactgt acatataacc actggtttta
     4981 tatacagcag tactgtacat ataaccactg gttttatata cagcagtcga cgtactgtac
     5041 atataaccac tggttttata tacagcagta ctgtacatat aaccactggt tttatataca
     5101 gcagtcgagg taagattaga tatggatatg tatatggata tgtatatggt ggtaatgcca
     5161 tgtaatatgc tcgactctag gatcttcgca agacccttcc tctatataag gaagttcatt
     5221 tcatttggag aggacacgct gaagctagtc gactctagcc tcgaggcgcg ccaggcctac
     5281 gcgtttaatt aaatgctcgc ctcttcccgt accgccgctg ccagcgtgca gcgcatgtcg
     5341 agtcggtacg ttgtcgtcgt gacgatgttg gtccaaacgc gaacgatcga acgaagcaac
     5401 tagcgcgggg ggtcgtgaac tagaagagaa aaaaaggcac aaactcgcgg tgttccccga
     5461 aacccctcac caacgtcagg atctgttcga ctctcgacaa attcctttgt atcctttctg
     5521 ttttgtgtac aataactcac gttcctattg gctttttcgt ctcgccctcc tagtgccttc
     5581 cacgcctcct ctttgaccga agcaggctcc acattacgcc tgctcatctc tgactcttac
     5641 gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag
     5701 cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc gcagaacccg
     5761 tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt
     5821 agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc
     5881 aagccggagt acgataaaac tatctccacg tcgattgtgc tcaatgcgct gaacgcgctc
     5941 ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgt cgaaggcgac
     6001 cgcaaagtct caggctcggc ctatcgcgaa accaaagatc gcggcttcca ccacggcacc
     6061 ttgctactca atgccgacct cagccgcctg gcaaactatc tcaatccgga taaaaagaaa
     6121 ctggcggcga aaggcattac gtcggtacgt tcccgcgtga ccaacctcac cgagctgttg
     6181 ccggggatca cccatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
     6241 ggcgagcgcg tggaagcgga aatcatctcc ccgaacaaaa cgccagactt gccaaacttc
     6301 gccgaaacct ttgcccgcca gagtagctgg gaatggaact tcggtcaggc tccggcattc
     6361 tcgcatctgc tggatgaacg ctttacctgg ggcggcgtgg aactgcattt cgacgttgaa
     6421 aaaggccata tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc gccgctggaa
     6481 gccctcgccg gacgactgca aggctgcctg taccgcgcag atatgctgca acaggagtgc
     6541 gaagcgctgt tggttgactt cccggaacag gaaaaagagc tacgggagtt atcggcatgg
     6601 atggcggggg ctgtaaggta cccgtatgat gttccggatt acgctggcta tccctacgac
     6661 gtgcccgact atgccgggta cccctatgac gtcccagact acgcagtgag caagggcgag
     6721 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     6781 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     6841 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     6901 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     6961 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     7021 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     7081 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     7141 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     7201 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     7261 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     7321 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     7381 gccgccggga tcactctcgg catggacgag ctgtacaagt agtgtacccg gggatcctct
     7441 agagtcgacc tgcaggcatg caagcttcag aagcgtgcta tcgaactcaa ccagggacgt
     7501 gcggcacaaa tgggcatcct tgctctcatg gtgcacgaac agttgggagt ctctatcctt
     7561 ccttaaaaat ttaattttca ttagttgcag tcactccgct ttggtttcac agtcaggaat
     7621 aacactagct cgtcttcacc atggatgcca atctcgccta ttcatggtgt ataaaagttc
     7681 aacatccaaa gctagaactt ttggaaagag aaagaatatc cgaatagggc acggcgtgcc
     7741 gtattgttgg agtggactag cagaaagtga ggaaggcaca ggatgagttt tctcgagaca
     7801 taccttcagc gtcgtcttca ctgtcacagt caactgacag taatcgttga tccggagaga
     7861 ttcaaaattc aatctgtttg gacctggata agacacaaga gcgacatcct gacatgaacg
     7921 ccgtaaacag caaatcctgg ttgaacacgt atccttttgg gggcctcccg ctacgacgct
     7981 cgctccagct ggggcttcct tactatacac agcgcgcata tttcacggtt gccagatgtc
     8041 aagacaattg gtttatgaaa acgtttaata tctcgcaaca agaccttgaa cttgtcgaag
     8101 tcgcaacgga aaaaattacg atgctttacg aagacaataa acatcatgtc ggagcagcaa
     8161 ttcgaacgaa aacgggagaa attatttcgg cagtccatat tgaagcatac attggacgag
     8221 tcacggtctg cgcagaagca attgcaattg gatcggcagt ctcgaatgga caaaaagact
     8281 ttgacacgat tgtcgcagtc cgacatccat actcggacga agtcgatcga tcgattcgag
     8341 tcgtctcgcc atgcggaatg tgccgagaac ttatttcgga ctacgcacca gactgctttg
     8401 tccttattga aatgaatgga aaacttgtca aaacgacgat tgaagaactt attccactta
     8461 aatacacgcg aaattgaaaa ccaggactga accttcctta aaaatttaat tttcattagt
     8521 tgcagtcact ccgctttggt ttcacagtca ggaataacac tagctcgtct tcaccatgga
     8581 tgccaatctc gcctattcat ggtgtataaa agttcaacat ccaaagctag aacttttgga
     8641 aagagaaaga atatccgaat agggcacggc gtgccgtatt gttggagtgg actagcagaa
     8701 agtgaggaag gcacaggatg agttttctcg aggccggtct ccctatagtg agtcgtatta
     8761 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8821 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8881 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8941 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9001 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9061 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9121 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9181 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9421 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9901 gctgcaatg
//